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Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs
Nucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to …
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A one-pot, whole-cell biocatalysis approach for vanillin production using lignin oil
Vanillin is a popular and versatile flavor compound, almost entirely produced from petroleum-derived phenol by a multi-step chemical …
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Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening
Developing therapeutic antibodies is laborious and costly. Here we report a method for antibody discovery that leverages the Illumina …
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Curse and Blessing of Non-proteinogenic Parts in Computational Enzyme Engineering
Enzyme engineering aims to improve or install a new function in biocatalysts for applications ranging from chemical synthesis to …
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The Expanded Central Dogma: Genome Resynthesis, Orthogonal Biosystems, Synthetic Genetics
Synthetic biology seeks to probe fundamental aspects of biological form and function by construction [i.e., (re)synthesis] rather than …
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New chemistries and enzymes for synthetic genetics
Beyond the natural nucleic acids DNA and RNA, nucleic acid chemistry has unlocked a whole universe of modifications to their canonical …
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Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases
Monooxygenases (MOs) face the challenging reaction of an organic target, oxygen and a cofactor – most commonly heme or flavin. To …
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Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and …
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Baeyer–Villiger monooxygenases: tunable oxidative biocatalysts
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical industry a poor public reputation, despite their …
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Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila
Among nature’s arsenal of oxidative enzymes, cytochrome P450s (CYPs) catalyze the most challenging reactions, the hydroxylations …
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