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New chemistries and enzymes for synthetic genetics
Beyond the natural nucleic acids DNA and RNA, nucleic acid chemistry has unlocked a whole universe of modifications to their canonical …
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Beyond active site residues: overall structural dynamics control catalysis in flavin-containing and heme-containing monooxygenases
Monooxygenases (MOs) face the challenging reaction of an organic target, oxygen and a cofactor – most commonly heme or flavin. To …
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Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and …
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Baeyer–Villiger monooxygenases: tunable oxidative biocatalysts
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical industry a poor public reputation, despite their …
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Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila
Among nature’s arsenal of oxidative enzymes, cytochrome P450s (CYPs) catalyze the most challenging reactions, the hydroxylations …
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Stabilization of cyclohexanone monooxygenase by computational and experimental library design
Enzymes often by far exceed the activity, selectivity, and sustainability achieved with chemical catalysts. One of the main reasons for …
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Switching the post-translational modification of translation elongation factor EF-P
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical industry a poor public reputation, despite their …
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Side-chain pruning has limited impact on substrate preference in a promiscuous enzyme
Detoxifying enzymes such as flavin-containing monooxygenases deal with a huge array of highly diverse xenobiotics and toxic compounds. …
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Exploring the substrate scope of Baeyer–Villiger monooxygenases with branched lactones as entry towards polyesters
Baeyer–Villiger monooxygenases (BVMOs) are biocatalysts that are able to convert cyclic ketones into lactones by the insertion of …
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Experimental protocols for generating focused mutant libraries and screening for thermostable proteins
Many proteins are rapidly deactivated when exposed to high or even ambient temperatures. This cannot only impede the study of a …
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