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Thermostability
Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and …
Friso S Aalbers
,
Maximilian JLJ Fürst
,
Stefano Rovida
,
Milos Trajkovic
,
J Rubén Gómez Castellanos
,
Sebastian Bartsch
,
Andreas Vogel
,
Andrea Mattevi
,
Marco W Fraaije
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Stabilization of cyclohexanone monooxygenase by computational and experimental library design
Enzymes often by far exceed the activity, selectivity, and sustainability achieved with chemical catalysts. One of the main reasons for …
Maximilian JLJ Fürst
,
Marjon Boonstra
,
Selle Bandstra
,
Marco W Fraaije
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DOI
Experimental protocols for generating focused mutant libraries and screening for thermostable proteins
Many proteins are rapidly deactivated when exposed to high or even ambient temperatures. This cannot only impede the study of a …
Maximilian JLJ Fürst
,
Caterina Martin
,
Nikola Lončar
,
Marco W Fraaije
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